photoshop has a slice tool that allows you to cut stuff up, and when you export for web, exports all individual images. i think most competing software would probably have that too.
It's a feature that's not mentioned too often as people get caught up in facebook/twitter/gamecenter. But support for spritesheets would be a great memory saver on some games.
Follow up question: is there a way to do a mass autocrop? I don't know if that's a word, but taking away the transparent holes in lots of images at a time is what I mean.
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